Reporter

Part:BBa_K1051000

Designed by: Xiang LI   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-07-02)

Stop codon free RFP in RFC[23] standard

Red fluorescent protein with assembly standard 23 is engineered mutant of red fluorescent protein from Discosoma striata (coral).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Modified

800px-Remove_codon.jpg Fluorescence proteins have been modified to RFC[23], and stop codons have been removed for fusion protein purpose.

Result

Reporter.jpg

[edit]
Categories
Parameters
emission607nm
n/aStop codon free RFP in RFC[23] standard