Part:BBa_K1036002

gfp (with LVA-tag) under plux R control

Description

The lux pL controlled luxR with lux pR is known as quorum sensing promoter(BBa_F2621). the lux pR is at slightly activated under normal circumstance and produces LuxI protein that synthesize a kind of acyl-homoserine lactone (AHL), which is a small molecule that can diffuse across the cell membrane and mediate intercellular coupling when it reaches the threshold as enough biomass accumulated. AHL will bind intracellular protein LuxR, which is also consecutively produced by luxR gene. The LuxR-AHL complex can activate the luxI promoter, and the positive feedback loop is built. That means once the biomass of bacteria reach the threshold, not only the expression of luxI protein gene will be increased. As for the GFP in this part, it still lacks a promoter to adjust its expression, so we added the quorum sensing promoter before it and it turned out to be Part:BBa_K1036003.

Experimental Data

Fig.1 showed the correct result of this part construction and Fig.2 showed the distinguish of BBa_K1036002 which was without lux promoter and BBa_K1036003 which was under lux promoter control.





Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2743
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 662
  Illegal BsaI.rc site found at 920
  Illegal BsaI.rc site found at 2023