Composite

Part:BBa_K1023006

Designed by: Michiel Karrenbelt   Group: iGEM13_Wageningen_UR   (2013-09-17)

actinGFP

GFP is fused to the n-terminus of actin in order to visualize the actin cytoskeleton. The actin originates from Aspergillus niger. Furthermore an inducable promoter is used which can be induced by xylose.


Usage and Biology

This construct is used to visualize the actin cytoskeleton by induction with xylose. This is done to be able to observe the structure and movements under a fluorescent microscope.

Actin cytoskeleton wrapping around an organelle

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1308
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1613
    Illegal XhoI site found at 2068
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1349
    Illegal AgeI site found at 1807
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1735
    Illegal BsaI.rc site found at 2188


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Parameters
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