Part:BBa_K1023002

xlnD terminator direction Forward forward_efficiency -NA- reversed_version -NA- reverse_efficiency -NA-

xlnD is a known forward terminator that is situated downstream of a gene encoding β-xylosidase from Aspergillus niger (GS1 strain) (Amaro-Reyes A et. al. 2011).
We have integrated this terminator downstream of ATP-sensor (BBa_K1023003) and eforRed (BBa_K1023005) genes, in which both of them are expressed under a constitutive promoter pkiA (BBa_K1023001), see figures below

Fig.1 Transformant (left) A. niger proved that the pkiThe red mycelium was clearly visible with eforRed upstream of xlnD terminator. The negative control (non codon-optimized eforRed) didn't have any colour.

Fig.2 ATP biosensor expression in A.niger

Secondary Structure

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Measurement

• [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

References

Amaro-Reyes A, García-Almendárez BE, Vázquez-Mandujano DG, Amaya-Llano S, Castaño-Tostado E, Guevara-González RG, Loera O, Regalado C.Homologue expression of a β-xylosidase from native Aspergillus niger.