Composite

Part:BBa_K1022128

Designed by: Maithili Krishnan   Group: iGEM13_TU-Delft   (2013-09-20)

pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly

This biobrick is the one of the three final Intergrated constructs of the project Peptidor by Team TU Delft iGEM 2013. This has a Promoter pBAD with the AIP receiver to sense the AIPs followed by a His6 SUMO tagged peptide along with a TetR which suppresses the timer module containing promoter pTET, cI is produced which suppresses pcI. Then Ulp protease is produced following by a Kill switch lysis device.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 2317
    Illegal NheI site found at 4609
    Illegal NheI site found at 6704
    Illegal NheI site found at 6727
    Illegal NotI site found at 5305
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 521
    Illegal BglII site found at 2441
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1405
    Illegal BamHI site found at 5274
    Illegal XhoI site found at 5283
    Illegal XhoI site found at 5314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2626
    Illegal AgeI site found at 6309
    Illegal AgeI site found at 6379
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 6960

Information

This part is one of the final constructs designed in the project [http://2013.igem.org/Team:TU-Delft/Project Peptidor] by Team TU Delft

Please visit [http://2013.igem.org/Team:TU-Delft iGEM 2013 Team TU Delft] for more



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