Part:BBa_K4693007

cDNA Serotonin 2

This is a short complementary DNA sequence that binds to our 44-mer serotonin aptamer(BBa_K4693000). Together with the aptamer, it forms an aptasensor that can quantify serotinin. This version of the cDNA was designed based on the kD value of the aptamer; cDNA pair compared to the kD value of the serotonin binding with the 44-mer aptamer. Multiple complementary sequences were designed to find a sequence with a higher kD value than -30.1 that would not affect the binding and formation of the designed FRET system. This chosen 12-mer cDNA had a kD value of -9.9 kcal/mol and the possible conformation didn’t affect the binding position of the cDNA hence the interaction of the fluorophore quencher pair.