Composite

Part:BBa_K4179020

Designed by: Baraah Rashed   Group: iGEM22_Technion-Israel   (2022-10-06)
Revision as of 05:20, 8 October 2022 by Baraah ra (Talk | contribs)


Luc_Blast_OraCell

The composite part comprises a Blasticidin S deaminase(BBa_K1943015), which is a Blasticidin resistance gene with the Kozak sequence. Downstream to this under the CMV(BBa_I712004) which is a constitutive promoter, an SV40PolyA terminator(BBa_K4235020) is cloned. Together in this composite, a cTNT promoter(BBa_K4179021) is found, downstream to the 8XGTIIC motifs(BBa_K4179017) followed by the luciferase gene(BBa_I712019) and the low most part SV40 Poly(A)(BBa_K4235020). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 213
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1090


[edit]
Categories
//awards/composite_part/nominee
Parameters
None