Part:BBa_K929104

Anti-GFP Nanobody

General information

Anti-GFP Nanobody
BioBrick Nr.	BBa_929104
RFC standard	RFC 25
Requirement	pSB1C3
Source	Gene synthesis by GeneArt
Submitted by	[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]

The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and shows a Kd value of 1.4 nM. Its CDR3 loop is very short and has significantly fewer contacts with the GFP ligand compared to other nanobodies. Furthermore, the shortness of this CDR3 loop leads to the exposure of the framework 2 region, which has a major contribution to the binding with GFP.

Characterization

For characterization and further applications see BBa_K929107.

[http://2016.igem.org/Team:Freiburg Team Freiburg 2016] improved this part for bacterial expression by including the pelB leader sequence in the gene. (see: BBa_K2114998)
(--Wladislaw Stroukov 17:32, 12 October 2016 )

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]