Part:BBa_K4760008

Lpp-OmpA-2xEAAAAK

Lpp-OmpA-2xEAAAAK is an improvement on Lpp'OmpA or Lpp-OmpA, a commonly used scaffold for bacteria outer membrane surface display. Addition of a rigid linker is thought to faciliate the display efficiency of smaller-size proteins of interest.

Usage and Biology

The usage of Lpp-OmpA(BBa_K2302003) vector can date to decades ago, when it was first invented to be a regular research tool for Gram- bacterial outer membrane. Lpp refers to lipoprotein, which is embedded in the outer membrane; followed by it is a reduced-in-size OmpA(BBa_K103006) variant, the N-terminus signal peptide and C-terminus chain to form the last part of the beta-barrel all cut off. The protein of interest linked on the C terminus of this fused protein, with Lpp and OmpA determining its subcellular location and direction.¹

By linking a EAAAAKEAAAAK rigid linker on the C-terminus, we expect to generate a better display effect for minor-scale protein of interest.

Reference

1.Charles F. Earhart, ‘[30] Use of an Lpp-OmpA Fusion Vehicle for Bacterial Surface Display’, in Methods in Enzymology, vol. 326 (Elsevier, 2000), 506–16, https://doi.org/10.1016/S0076-6879(00)26072-2.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 55
  
• 1000
  COMPATIBLE WITH RFC[1000]