Coding

Part:BBa_K4760004

Designed by: Xianghai Jiang   Group: iGEM23_SJTU-BioX-Shanghai   (2023-10-09)


Lpp-OmpA-2xEAAAAK-His10

This is a variation of the reported Lpp'OmpA-His6 of Alves et al,1 possessing the same function of help recovering OMVs with nickel. The scaffold of Lpp'OmpA together with a flexible linker GGGGS would display a 10 aa His-Tag on the surface of Gram- bacteria outer membrane or OMVs, giving it Ni-binding affinity.

References

1.Alves NJ, Turner KB, DiVito KA, Daniele MA, Walper SA. Affinity purification of bacterial outer membrane vesicles (OMVs) utilizing a His-tag mutant. Res Microbiol. 2017;168(2):139-146.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None