Tag

Part:BBa_K4760002

Designed by: Xianghai Jiang   Group: iGEM23_SJTU-BioX-Shanghai   (2023-10-09)


flexible GGGGS-His10 tag

This tag is designed under the idea that too large scaffold may mask the His6 tag, we used a higher-affinity his10 tag linked behind flexible linker GGGGS, which usually helps to avoid unfavorable secondary structure formation. Using this tag, we constructed the composite part BBa_K4760003.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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