Coding

Part:BBa_K4760001

Designed by: Xianghai Jiang   Group: iGEM23_SJTU-BioX-Shanghai   (2023-10-09)


SpyTag003-3xflag

This part is the reportedly improved SpyTag variant, SpyTag003, which can rapidly form isopeptide bond with SpyCatcher003.

Usage and Biology

The original version of SpyTag and SpyCatcher are naturlly isopeptide forming counterparts, registered as BBa_K1159201 and BBa_K1159200. SpyTag003 and SpyCatcher003 are optimized by Keeble et al 1, and here we utilize them in our composite parts (reserved).

References

1.Keeble AH, Turkki P, Stokes S, et al. Approaching infinite affinity through engineering of peptide-protein interaction. Proc Natl Acad Sci U S A. 2019;116(52):26523-26533.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None