Coding
Part:BBa_K4760001
Designed by: Xianghai Jiang Group: iGEM23_SJTU-BioX-Shanghai (2023-10-09)
SpyTag003-3xflag
This part is the reportedly improved SpyTag variant, SpyTag003, which can rapidly form isopeptide bond with SpyCatcher003.
Usage and Biology
The original version of SpyTag and SpyCatcher are naturlly isopeptide forming counterparts, registered as BBa_K1159201 and BBa_K1159200. SpyTag003 and SpyCatcher003 are optimized by Keeble et al 1, and here we utilize them in our composite parts (reserved).
References
1.Keeble AH, Turkki P, Stokes S, et al. Approaching infinite affinity through engineering of peptide-protein interaction. Proc Natl Acad Sci U S A. 2019;116(52):26523-26533.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |