Reporter

Part:BBa_K3013002

Designed by: Gereon Fischer   Group: iGEM19_Aachen   (2019-10-13)


FbFP BS2

FbFP BS2 is a flavin mononucleotide-based fluorescent protein containing a LOV domain. It can function as a reporter in anaerobic systems, for example as an in vivo detection system for expression of magnetosomes under anaerobic conditions (see BBa_K3013003).

Color: cyan-green
Excitation maximum: 449 nm
Emission maximum: 495 nm


Usage and Biology

This part was used in the composite part BBa_K3013003.
This part was never expressed on its own. For more information about expression of this part and fluorescence measurements see BBa_K3013003.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 255
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/reporter
//function/reporter/fluorescence
Parameters
emission
excitation
protein