DNA

Part:BBa_K2566006

Designed by: Martin Holmes   Group: iGEM18_CCA-San_Diego   (2018-10-12)

Degrade Chrysene into Acetyl-A

CCA iGEM 2019 used Gibson assembly to clone this part, which we split into three fragments for synthesis. You can see the Experiments tab of CCA_San_Diego 2019's wikifor more information on Gibson primers and colony PCR primers that correspond to this part. Experimental settings were designed so that chrysene was the only available carbon source. As you can see in Figure 1, whereas the control strain was unable to utilize chrysene as a carbon source, the strain in which Part:BBa K2566006 was cloned is able to metabolize chrysene and grow using chrysene as a sole carbon source.

ChryseneResults

Figure 1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
    Illegal NheI site found at 226
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 48
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1354
    Illegal AgeI site found at 3480
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 688
    Illegal SapI.rc site found at 4919
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