Coding

Part:BBa_K2306012

Designed by: Jeroen Jacques   Group: iGEM17_TUDelft   (2017-10-13)


Cas13a with purification tags

This BioBrick is a sequence encoding the gene for the protein Cas13a (Gootenberg et al. 2017) including tags for protein purification. It does not include the double repeat/spacer sequence to define the target of the Cas13a, but only encodes for the protein itself. It can be used in combination with BBa_K2306013 which is the double repeat/spacer sequence unique to Cas13a. Cas13a is a protein that, upon recognition of its target RNA sequence which is encoded in the spacer sequence, cleaves the target RNA and then engages in collatoral cleavage of RNA in its surroundings in an aspecific manner.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 260
    Illegal BglII site found at 845
    Illegal BglII site found at 2006
    Illegal BglII site found at 2480
    Illegal BamHI site found at 1
    Illegal BamHI site found at 451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None