Part:BBa_K1963000
Hfq from E. coli K-12
Hfq is an RNA binding protein. It can bind to a range of RNA sequences but is often used in conjunction with an RNA hairpin formed by the E. coli micC sequence. A bbobrick containing a template for making sRNA fusions to micC has also been designed BBa_K1963002.
Usage and Biology
The Hfq (Host Factor Q) protein forms a homo-hexameric ring structure that contains several RNA binding sites. Hfq has various modes of action, but Hfq-mediated regulation is can be achieved through either modulation of RNA susceptibility to RNases; or regulation of accessibility to the ribosome binding sites (RBS); or by facilitating RNA-RNA interactions. In bacteria, small RNA molecules (sRNAs) usually interact directly with Hfq and are often partially complementary to certain target mRNAs.
Figure 1: E. coli Hfq based on PDB file 2YHT
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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