Composite

Part:BBa_K1584036

Designed by: Nu Ri Choi, Seung Meen Choi, SOOJI LEE, Brittany LEE, Hyun Min Park   Group: iGEM15_Elan_Vital_Korea   (2015-09-13)


LuxR-responsive promoter with LacZ CDS

This part contains two CDSs for expression in bacteria. The first is LuxR which is controlled by a constitutive left and a right promoter. The second is LacZ which is controlled by promoter with a LuxR recognition site. LuxR protein forms a complex with quorum sensing signalling molecule homoserine lactone,which then increases the rate of transcription. Therefore,in the presence of homoserine lactone, the LacZ reporter is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


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Parameters
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