Reporter
Part:BBa_K1584035
Designed by: Seung Meen Choi, Brittany Lee, Nu Ri Choi, SOOJI LEE, Hyun Min Park Group: iGEM15_Elan_Vital_Korea (2015-08-31)
Constitutive LuxR plus AHL-inducible LacZ
It produces two proteins, first LuxR and then LacZ. LuxR is expressed constitutively. When AHL quorum sensing molecule is present, it activates LuxR. Then, the resulting AHL-LuxR compound would bind to the LuxpR promoter, producing LacZ. LacZ encodes the alpha fragment of beta galactosidase and, in the presence of X-gal, can produce a blue colored molecule in DH5alpha, DH10B and Top10 bacterial strains.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 908
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Categories
Parameters
None |