Gas Vesicle polycistonic gene
This 6 kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Promotion of the sequence results in expression of gas vesicles, organelles made entirely out of protein. These organelles contain gas and therefore provide bouyancy to the cell. This slows the rate of settling of the cells in Saline. For more info on gas vesicles, see Walsby 1994.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 334
Illegal BamHI site found at 4139
Illegal XhoI site found at 3827
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1187
Illegal AgeI site found at 4106
- 1000COMPATIBLE WITH RFC
Electron Micrograph of Gas Vesicles in E.coli protoplasts
The transfer of the GVP gene cluster to E. coli was done first by Ning Li and Maura C. Cannon:
Ning Li and Maura C. Cannon (May 1998). "Gas Vesicle Genes Identified in Bacillus megaterium and Functional Expression in Escherichia coli". Journal of Bacteriology 180(9): 2450–2458.