Plasmid

Part:pSB5AC5

Designed by: Reshma Shetty   Group: BE.109 Instructors   (2006-03-13)


pSB5AC5-P1011.I50020

This is a single copy vector designed entirely from BioBrick parts. See http://openwetware.org/wiki/Synthetic_Biology:Vectors/Single_copy_plasmid for details.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6731
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6731
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6737
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6731
    Illegal BglII site found at 4283
    Illegal BglII site found at 6350
    Illegal BglII site found at 7203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6731
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6731
    Illegal XbaI site found at 6746
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1877
    Illegal NgoMIV site found at 5793
    Illegal AgeI site found at 3546
    Illegal AgeI site found at 4439
    Illegal AgeI site found at 5669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2692
    Illegal SapI.rc site found at 3689
    Illegal SapI.rc site found at 4899


[edit]
Categories
Parameters
None