Deleted
Sample Discontinued
Experience: None
Not Used
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Plasmid

Part:pSB4AC5

Designed by: Reshma Shetty   Group: BE.109 Instructors   (2006-03-17)


pSB4AC5-P1011.I50020

Low copy vector designed entirely from BioBricks parts. See http://openwetware.org/wiki/Synthetic_Biology:Vectors/Wishlist for details.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4478
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4478
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4484
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4478
    Illegal BamHI site found at 4514
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4478
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4478
    Illegal XbaI site found at 4493
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2100
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4843
    Illegal BsaI.rc site found at 1212


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