Plasmid_Backbone
pSB2K4

Part:pSB2K4

Designed by: Austin Che, Leon Chan and Sri Kosuri   Group: Knight Lab   (2006-01-19)

pSB2K3-derived BioBrick plasmid free of many restriction sites

Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric recognition sites have been removed, and some non-symmetric sites have also been removed including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. See pSB2K3 for more information. Note that the VF2 site has a single base mutation in the middle of it. However, VF2 works well as a primer for PCR or sequencing with this plasmid.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4446
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4446
    Illegal BglII site found at 1645
    Illegal BglII site found at 2692
    Illegal BamHI site found at 2566
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4446
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4446
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4461
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 23
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4446
    Illegal XbaI site found at 4461
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/inducible
//plasmidbackbone/operation
//plasmidbackbone/version/4
Parameters
chassislacIq like BBa_V1003
copies<10 or >100
mcsBioBrick
originF' and P1 lytic
resistanceK