Plasmid_Backbone
pSB2K0

Part:pSB2K0

Designed by: Brookhaven National Lab   Group: Registry   (2005-06-13)


pSB2K0 (pSCANS-BNL; Replaced by pSB2K3)

Warning, this plasmid does not have a proper BioBrick cloning site.

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1024
    Illegal XbaI site found at 2574
    Illegal SpeI site found at 1052
    Illegal PstI site found at 1745
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1024
    Illegal NheI site found at 4319
    Illegal SpeI site found at 1052
    Illegal PstI site found at 1745
    Illegal NotI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1024
    Illegal BglII site found at 2620
    Illegal BglII site found at 3667
    Illegal BamHI site found at 1046
    Illegal XhoI site found at 30
    Illegal XhoI site found at 1013
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1024
    Illegal XbaI site found at 2574
    Illegal SpeI site found at 1052
    Illegal PstI site found at 1745
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1024
    Illegal XbaI site found at 2574
    Illegal SpeI site found at 1052
    Illegal PstI site found at 1745
    Illegal NgoMIV site found at 2063
    Illegal NgoMIV site found at 4305
    Illegal AgeI site found at 1939
    Illegal AgeI site found at 3598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1024
    Illegal XbaI site found at 2574
    Illegal SpeI site found at 1052
    Illegal PstI site found at 1745


[edit]
Categories
//classic/plasmid/uncategorized
//plasmidbackbone/archive
Parameters
chassis
copies<10 or >100
insertNone
mcsNonstandard
origin
resistanceK