Part:pSB1AC3-1

pSB1AC3 with ccdB insert

Target plasmid for three antibiotic assembly. Chloramphenicol resistant. Contains an insert having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. Grown in the special strain DB3.1, which is ccdB resistant.

Usage and Biology

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Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 699
  Illegal EcoRI site found at 2
  Illegal XbaI site found at 17
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2
  Illegal SpeI site found at 700
  Illegal PstI site found at 714
  Illegal NotI site found at 8
  Illegal NotI site found at 707
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2
  Illegal BamHI site found at 687
  Illegal XhoI site found at 1733
  Illegal XhoI site found at 2625
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 700
  Illegal EcoRI site found at 2
  Illegal XbaI site found at 17
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2
  Illegal XbaI site found at 17
  Illegal SpeI site found at 700
  Illegal PstI site found at 714
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 137
  Illegal BsaI.rc site found at 2771
  

Functional Parameters

chassis	DB3.1
copies	100-300
insert	ccdB
mcs	Biobrick
origin	pMB1
resistance	AC