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Plasmid

Part:pSB1AC3-1

Designed by: Tom Knight   Group: iGEM_Registry   (2005-06-07)


pSB1AC3 with ccdB insert

Target plasmid for three antibiotic assembly. Chloramphenicol resistant. Contains an insert having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. Grown in the special strain DB3.1, which is ccdB resistant.

Usage and Biology

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 699
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 17
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2
    Illegal SpeI site found at 700
    Illegal PstI site found at 714
    Illegal NotI site found at 8
    Illegal NotI site found at 707
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2
    Illegal BamHI site found at 687
    Illegal XhoI site found at 1733
    Illegal XhoI site found at 2625
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 700
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 17
    Illegal SpeI site found at 700
    Illegal PstI site found at 714
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 137
    Illegal BsaI.rc site found at 2771

Functional Parameters

chassisDB3.1
copies100-300
insertccdB
mcsBiobrick
originpMB1
resistanceAC
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Categories
//classic/plasmid/uncategorized
Parameters
chassisDB3.1
copies100-300
insertccdB
mcsBiobrick
originpMB1
resistanceAC