Coding

Part:BBa_K2023009

Designed by: Célia Chenebault   Group: iGEM16_Ionis_Paris   (2016-10-14)


Gaussia luciferase coding sequence optimized for use in E.coli DH5-Alpha

This biobrick is the Gaussia Luciferase with 6X-His tag for easier protein purification. It is codon optimized for E.coli DH5-alpha.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 94
    Illegal NheI site found at 505
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/light
Parameters
proteinGaussia Luciferase