T7

Part:BBa_Z0504

Designed by: Sriram Kosuri   Group: T7.2   (2006-06-28)


T7.2 Design Revision D

Fixed gene 3 errors, removed restriction sites from Z0301 http://openwetware.org/wiki/T7.2

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 8650
    Illegal XbaI site found at 11749
    Illegal XbaI site found at 31530
    Illegal SpeI site found at 15441
    Illegal PstI site found at 14987
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 8650
    Illegal NheI site found at 20117
    Illegal SpeI site found at 15441
    Illegal PstI site found at 14987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 8650
    Illegal BglII site found at 10355
    Illegal BamHI site found at 8473
    Illegal XhoI site found at 16445
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 8650
    Illegal XbaI site found at 11749
    Illegal XbaI site found at 31530
    Illegal SpeI site found at 15441
    Illegal PstI site found at 14987
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 8650
    Illegal XbaI site found at 11749
    Illegal XbaI site found at 31530
    Illegal SpeI site found at 15441
    Illegal PstI site found at 14987
    Illegal NgoMIV site found at 12185
    Illegal AgeI site found at 1086
    Illegal AgeI site found at 32929
    Illegal AgeI site found at 34670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1137
    Illegal BsaI site found at 7317
    Illegal BsaI site found at 13233
    Illegal BsaI site found at 20405
    Illegal BsaI site found at 22980
    Illegal BsaI site found at 23619
    Illegal BsaI site found at 26763
    Illegal BsaI.rc site found at 6567
    Illegal BsaI.rc site found at 8554
    Illegal BsaI.rc site found at 9071
    Illegal BsaI.rc site found at 10453
    Illegal BsaI.rc site found at 11525
    Illegal BsaI.rc site found at 12841
    Illegal BsaI.rc site found at 14882
    Illegal SapI.rc site found at 8789
    Illegal SapI.rc site found at 13313
    Illegal SapI.rc site found at 34261


[edit]
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