Part:BBa_Z0502

Bacteriophage T7.2 -- design optimization

Design of T7.2 with Venus YFP and removed secondary structure for strong RBS overlap.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 8650
  Illegal XbaI site found at 11749
  Illegal XbaI site found at 31530
  Illegal SpeI site found at 15441
  Illegal PstI site found at 14987
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 8650
  Illegal NheI site found at 20117
  Illegal SpeI site found at 15441
  Illegal PstI site found at 14987
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 8650
  Illegal BglII site found at 10355
  Illegal BamHI site found at 8473
  Illegal XhoI site found at 16445
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 8650
  Illegal XbaI site found at 11749
  Illegal XbaI site found at 31530
  Illegal SpeI site found at 15441
  Illegal PstI site found at 14987
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 8650
  Illegal XbaI site found at 11749
  Illegal XbaI site found at 31530
  Illegal SpeI site found at 15441
  Illegal PstI site found at 14987
  Illegal NgoMIV site found at 12185
  Illegal AgeI site found at 1086
  Illegal AgeI site found at 32929
  Illegal AgeI site found at 34670
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1137
  Illegal BsaI site found at 7317
  Illegal BsaI site found at 13233
  Illegal BsaI site found at 20405
  Illegal BsaI site found at 22980
  Illegal BsaI site found at 23619
  Illegal BsaI site found at 26763
  Illegal BsaI.rc site found at 6567
  Illegal BsaI.rc site found at 8554
  Illegal BsaI.rc site found at 9071
  Illegal BsaI.rc site found at 10453
  Illegal BsaI.rc site found at 11525
  Illegal BsaI.rc site found at 12841
  Illegal BsaI.rc site found at 14882
  Illegal SapI.rc site found at 8789
  Illegal SapI.rc site found at 13313
  Illegal SapI.rc site found at 34261