Reporter

Part:BBa_Z0301

Designed by: Sriram Kosuri   Group: T7.2   (2006-06-21)

Venus YFP - E. coli codon optimized

Brighter Variant than standard EYFP.

Usage and Biology

Nucleotides 4-39 were codon-shuffled to minimize the predicted secondary structure of the RBS BBa_Z0261-CDS junction. Predicted ΔG of folding of RBS-CDS junction is -3.5 kCal/mol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 202


[edit]
Categories
//function/reporter/fluorescence
Parameters
None