Part:BBa_Z0170

T7.2 gene 1, codon-shuffled

Encodes the same protein as T7.2 gene 1, with first 12 codons after start shuffled to reduce RBS-CDS secondary structure. Based on BBa_Z0107.

Usage and Biology

(Copied from BBa_Z0107)

Approximately at position 2500 hundred is a possible E. coli promoter (S1). TTGAC is the consensus -35 region located at 2498-2502 the possible -10 region is TGTACT from 2520-2525

The GTTGAC from 2497-2502 encodes V-D dipeptide. Redesigned to be GTAGAT (~same tRNA abundance)

The GAT peptide from 2518-2520 an D amino acid is mutated to GAC to disrupt the -10 region.

(New)

Nucleotides 4-39 were redesigned to encode the same amino-acid sequence as the original sequence, but lead to less secondary structure with the preceding RBS (BBa_Z0262) in the T7.2 design.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]