Coding

Part:BBa_Z0156

Designed by: Sriram Kosuri   Group: Endy Lab   (2006-05-04)

T7.2 gene 14


Usage and Biology

Silent mutations on 3' end to remove Gene 15 RBS

Nucleotides 4-39 were codon-shuffled to minimize predicted RNA secondary structure at RBS BBa_Z0262-CDS junction. Predicted ΔG of folding of RBS-CDS junction is -5.4 kCal/mol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None