Coding

Part:BBa_Z0152

Designed by: Sriram Kosuri   Group: Endy Lab   (2006-04-27)


T7.2 gene 2.5

mutation from T7 to remove RBS from gene 2.8


Usage and Biology

Mutation to remove -35 region of S2 promoter. The -10 region is already significantly non-concensus, so no further changes were made.

Nucleotides 4-39 were codon-shuffled to minimize predicted secondary structure at the RBS BBa_Z0262-CDS junction. Predicted ΔG of RBS-CDS folding is -1.53kCal/mol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 361
    Illegal SapI.rc site found at 79


[edit]
Categories
Parameters
None