Coding

Part:BBa_Z0137

Designed by: Sri Kosuri   Group: Endy Lab   (2006-04-26)

T7.2 gene 19 (19.2, 19.3)

large terminase subunit. binds capsid pro-head. Also contains within the sequence for 19.2 and 19.3 within the gene.

Usage and Biology

Nucleotides 4-39 were codon-shuffled to minimize predicted RNA secondary structure at RBS [1]-CDS junction. Predicted ΔG of folding of RBS-CDS junction is -2.7 kCal/mol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 233


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Categories
Parameters
None