Coding

Part:BBa_Z0129

Designed by: Sri Kosuri   Group: Endy Lab   (2006-04-26)

T7.2 gene 12

a structural tail protein of the phage capsid; 6 copies per virion

Usage and Biology

Nucleotides 4-39 were codon-shuffled to minimize predicted RNA secondary structure at the RBS BBa_Z0262-CDS junction. Predicted ΔG of folding of the RBS-CDS junction is -1.7 kCal/mol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 980
    Illegal BsaI site found at 1619
    Illegal BsaI.rc site found at 112
    Illegal BsaI.rc site found at 1347


[edit]
Categories
Parameters
None