Coding

Part:BBa_Z0121

Designed by: Sri Kosuri   Group: Endy Lab   (2006-04-25)

T7.2 gene 5

T7 DNA Polymerase consists of two proteins, the gene 5 protein and the processivity factor E. coli thioredoxin. Thioredoxin forms a 1:1 complex with the T7 protein and increases its processivity by at least 1000-fold, the complex also increases the activity of the associated 5\'->3\' exonuclease on dsDNA but has little effect on ssDNA exonucleolytic activity.

Usage and Biology

Nucleotides 4-39 were codon-shuffled to minimize predicted RNA secondary structure at the RBS BBa_Z0261-CDS junction. Predicted ΔG of folding of the RBS-CDS junction is -4.3 kCal/mol.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2116
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2116
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 362
    Illegal BsaI.rc site found at 2011
    Illegal BsaI.rc site found at 2042
    Illegal SapI.rc site found at 442


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Parameters
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