Coding

Part:BBa_Z0120

Designed by: Sri Kosuri   Group: Endy Lab   (2006-04-25)

T7.2 gene 4A/4B

The full-length gene 4 protein is a 5\'->3\' helicase of the E. coli DnaB family and a DNA primase. An in-frame internal start site within gene 4 results in a protein, gp4B, that lacks the N-terminal 63 amino acids of the 63 kDa gp4A, and consequently lacks primase activity. The primase recognition sites on ssDNA are 5\'-G/TGGTC, and 5\'-GTGTC.


Usage and Biology

Nucleotides 4-39 were codon-shuffled to minimize predicted secondary structure at the RBS BBa_Z0262-CDS junction. Predicted ΔG of folding of the RBS-CDS junction is -4.6 kCal/mol.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1266
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1266
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1266
    Illegal NgoMIV site found at 1702
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1042


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Categories
Parameters
None