Coding

Part:BBa_Z0116

Designed by: Sri Kosuri   Group: Endy Lab   (2006-04-12)

T7.2 gene 2

Gp2 binds to and is an inhibitor of E. coli RNAP but in K-12 or B strains the defects due to a lack of gene 2 activity are reduced DNA replication and premature breakdown of replicating DNA, specifically at the left genome end where the major E. coli promoters are found.


Usage and Biology

Nucleotides 4-39 were codon-shuffled to minimize predicted secondary structure between the coding sequence and its associated RBS BBa_Z0261 in the T7.2 design. Predicted ΔG of folding of RBS-CDS junction is -3.1kCal/mol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 196
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 196
    Illegal BamHI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 196
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 100


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Categories
Parameters
None