Part:BBa_Z0115

T7.2 gene 1.2

Gene 1.2 inhibits E. coli dGTPase and converts it into a rGTP-binding protein, which is important for T7 only when dgt is over-expressed (optA1 mutation). T7 gene 1.2 also causes phage exclusion by the F plasmid, but T3 gene 1.2 overcomes the exclusion system.

Usage and Biology

gene 1.2 contains only 3 bp (taa) of the 23-bp consensus region of phi1.3. However, there is no need to mutate it, as the lack of the downstream region should be enough to prevent any promoter like activity.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]