Part:BBa_Z0101

T7 gene 0.3

Gene 0.3 is expressed at very high levels and protects T7 from various type I restriction systems by mimicking the shape of B DNA. Gp0.3 has been shown to bind the EcoKI restriction enzyme in a 2:1 complex and to displace the enzyme from its target DNA.

Second site suppressors of 0.7-,2- infections map to gene 0.3 (Molineux, PC). Unknown why this is the case.

Usage and Biology

inhibits EcoB and EcoK host restriction systems

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]