Part:BBa_Y00012
mitochondrial LYS12
S. cerevisiae gene LYS12 http://db.yeastgenome.org/cgi-bin/locus.pl?locus=lys12 is required for the fourth step of lysine biosynthesis. It is normally encoded by the nuclear genome and the protein is imported to the mitochondria where it oxidatively decarboxylates homo-isocitrate to alpha-ketoadipate. Strains deleted for LYS12 are viable but show decreased growth in the absence of lysine. Part Y00012 is the S. cerevisiae LYS12 gene that has been recoded for expression in the mitochondria, which uses a different genetic code and separate transcription/translation machinery for expression mitochondrial genes. The part is start to stop flanked by biobrick ends so has no promoter for expression or sequences for recombination into the mitochondria.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 9
Illegal suffix found in sequence at 1147 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
Illegal SpeI site found at 1148
Illegal PstI site found at 1162
Illegal NotI site found at 15
Illegal NotI site found at 1155 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
Illegal BamHI site found at 1069 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 9
Illegal suffix found in sequence at 1148 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 9
Illegal XbaI site found at 24
Illegal SpeI site found at 1148
Illegal PstI site found at 1162 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 399
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