Cell

Part:BBa_V1021

Designed by: Meagan Lizarazo   Group: Arkin Lab   (2006-11-16)


MachI

Taken from:http://openwetware.org/wiki/E._coli_genotypes#Mach1

ΔrecA1398 endA1 tonA Φ80ΔlacM15 ΔlacX74 hsdR(rK- mK+)

  • From Invitrogen
  • Doubling time approx. 50 min and supposedly fastest growing chemically competent cloning strain available
  • Mach1 cells are derivatives of E. coli W strains (ATCC 9637, S. A. Waksman), rather than E. coli K-12. This may have implications for BL-1 status for some facilities (apparently not for MIT).
  • See Bloom04 patent for details on the construction and properties of this strain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
genotypeΔrecA1398 endA1 tonA Φ80ΔlacM15 ΔlacX74 hsdR(rK- mK+)