Cell
GH371

Part:BBa_V1011

Designed by: John Anderson   Group: Arkin Lab   (2006-06-29)

E. coli strain GH371; 5-fluorouracil resistant upp mutant of GeneHogs

GeneHogs is a phage-resistant variant of DH10B (see http://www.invitrogen.com/content/sfs/manuals/genehogs_man.pdf. GH371 is an upp mutant of GeneHogs generated by lambda-red recombination.

GH371 is resistant to ~10 ug/mL 5-Fluorouracil and has been used for negative genetic selections (See Chin and coworkers: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=Abstract&list_uids=16408021&query_hl=5&itool=pubmed_docsum


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
genotypeF- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2 (confers phage T1 resistance),upp-