Cell
JM109

Part:BBa_V1010

Designed by: Randy Rettberg   Group: Arkin Lab   (2005-08-30)

JM109

  • From [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/ecoli_genotypes.asp NEB]

Usage and Biology

  • Partly restriction-deficient; good strain for cloning repetitive DNA (RecA–).
  • Suppresses many amber mutations when glutamine is acceptable but not the S100 or S7 mutations of λ, e.g., λgt11.
  • Can also be used for M13 cloning/sequencing and blue/white screening.
  • Sigma lists e14-
  • nalidixic acid resistant

NOTE: This promoter driving the expression of lacI was sequenced in this strain using a primer in mhpR (upstream of lacI) and a primer in the opposite orientation in lacI. The lac promoter was found to be identical to wildtype. Thus, the -35 sequence was GCGCAA not GTGCAA as expected with lacIQ. Therefore this strain (or at least the version we have) does NOT appear to be lacIQ unless there is an undocumented extra copy of lacI] somewhere on the genome or F' plasmid. This result is somewhat confirmed by the fact that a lacI regulated promoter driving expression of YFP on a medium copy vector does not repress completely. -Reshma 13:48, 5 May 2005 (EDT)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
genotypeendA1, recA1, gyrA96, thi, hsdR17, (rk-, mk+), relA1, supE44, Δ(lac-proAB), [F', traD36, proAB, lacIqZΔM15]