Protein_Domain
VSV-G tag

Part:BBa_T2021

Designed by: Reshma Shetty   Group: iGEM07_Example   (2009-09-03)

VSV-G epitope tag tail domain (YTDIEMNRLGK)

This is a VSV-G epitope tag tail domain designed to be at the C-terminus of a protein. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins.

Usage and Biology

The VSV-G epitope tag is derived from the C-terminal residues of vesicular stomatitis virus glycoprotein (VSV-G). Proteins can be purified or quantitatively analyzed using a monoclonal antibody to the VSV-G tag. Elution from the antibody occurs at low pH.

The VSV-G is probably best used at the C-terminus of a protein of interest, but has also been used internally Battini.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/affinity
//proteindomain/tail
Parameters
None