Intermediate

Part:BBa_S05292

Designed by: Xudong Luo   Group: Guests   (2015-09-14)

Plac/ara-1+strong RBS+LacI(+LVA)

Construction intermediate

Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1275
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None