Intermediate

Part:BBa_S05063

Designed by: yue hu   Group: Guests   (2012-09-19)

R0084:B0034

This part contains a osmolarity sensing promoter R0084, combined with the strong RBS B0034.As this part is in pSB1C3, which has terminators bracketing its MCS , allowing a protein coding sequence to be inserted into this vector and be immediately ready for expression.At low osmolarity, the transcription is activated, while high osmolarity repressing transcription.Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None