Intermediate

Part:BBa_S04145

Designed by: Tom Tran   Group: Guests   (2008-10-28)

The Partial Butanol Operon (Parts I725021+I725022+I725023)

The partial butanol operon required for Clostridium acetobutylicum to convert acetyl-CoA to butanol. The operon includes the following butanol inserts:

  1. RBS + B-hydroxy butyryl CoA dehydrogenase
  2. RBS + Enoyl-CoA hydratase
  3. RBS + Butyryl CoA dehydrogenase

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 308
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 763
    Illegal AgeI site found at 1077
    Illegal AgeI site found at 1208
    Illegal AgeI site found at 1988
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None