Intermediate

Part:BBa_S03533

Designed by: Davidson and Missouri Western   Group: iGEM06_Davidson   (2006-08-02)

pBad-HixC-TetB-RBSrev

Construction intermediate


Usage and Biology

Interestingly, this intermediate conveys Tetracycline resistance to E. coli. Note there is no promoter other than what the vector brings with it. This indicates a "read through" transcription. We are using this intermediate part as a way to screen for ways to insulate the BioBrick parts from "read through" transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1215
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1069
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 515
    Illegal NgoMIV site found at 675
    Illegal NgoMIV site found at 1043
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None