Part:BBa_S03494

CD154 (CD40 Ligand)

CD154 is a protein that is normally expressed on the surface of T cells. It regulates B cell function by ligating to CD40, a receptor on the B cell surface. Stimulation of CD40 with CD154 promotes the proliferation and differentiation of naive B lymphocytes into plasma and memory B cells, facilitating a humoral response. The CD154-CD40 interaction generally also results in the upregulation of costimulatory molecules, the activation of antigen presenting cells, the priming of T cells, and the activation of T cell mediated effector functions. The CD154 protein provides us with a tool for stimulating the immune system, a mechanism that can be used to learn more about the specific interactions in the humoral response system and also to enhance the effectiveness of immunotherapy.

Usage and Biology

Some information regarding the role of CD154 as an immunostimulator:

"CD40 stimulation of human peripheral B lymphocytes: distinct response from naive and memory cells." (2003) by J.F. Fecteau and S. Neron, issued in "The Journal of Immunology," 171: 4621-4629.

"The role of CD40-CD154 interaction in cell immunoregulation." (2004) by Yufei Xu and Guoxing Song, issued in "Journal of Biomedical Science," 11:426-438 (DOI: 10.1159/000077892).

CD154 gene sequence was obtained from the National Center for Biotechnology Information website.

More information about research involving CD154 can be found online: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=full_report&list_uids=959

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1150
  Illegal XbaI site found at 1332
  Illegal PstI site found at 1749
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1150
  Illegal PstI site found at 1749
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1150
  Illegal BamHI site found at 962
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1150
  Illegal XbaI site found at 1332
  Illegal PstI site found at 1749
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1150
  Illegal XbaI site found at 1332
  Illegal PstI site found at 1749
  
• 1000
  COMPATIBLE WITH RFC[1000]