Intermediate

Part:BBa_S03491

Designed by: Katherine Aull   Group: BE.109 2006   (2006-05-18)


Constitutive generator of 3OC6HSL

Contains LuxI enzyme, which produces compound that de-represses luxPR promoter in the presence of LuxR.


Usage and Biology

LuxI is an enzyme which produces 3OC6HSL, the ligand of LuxR which allows it to leave the luxPR promoter and allow transcription. This part is a generator. It is intended to create LuxI in sufficient volume that 3OC6HSL can be manufactured by extraction from the culture.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None