Coding

Part:BBa_S03489

Designed by: Kalvin Kao   Group: BE.109 2006   (2006-05-18)


Influenza Virus NS1A Binding Protein

NS1-binding protein (NS1-BP) is a novel human protein that has been shown to be associated with cellular splicing apparatus. Specifically, NS1-BP is located in the nuclei of cells and is believed to promote pre-mRNA splicing. NS1 nonstructural protein from influenza A virus alters the function of NS1-BP and also inhibits pre-mRNA splicing. The NS1-BP mediates the splicing-inhibitory actions of NS1 by binding to it. For more general experimental purposes, NS1-BP could be used to detect infection by influenza A virus and more specifically, the presence of functional NS1 protein from an influenza A virus.


Usage and Biology

See: "NS1-Binding protein (NS1-BP): a novel human protein that interacts with the influenza A virus nonstructural NS1 protein is relocalized in the nuclei of infected cells." (1998) by T. Wolff, R.E. O'Neill, and P. Palese issued in "Journal of Virology", September 1998, Vol. 72, No. 9, pp. 7170-7180. Sequence was taken from the gene-centered information section of the National Center for Biotechnology Information website.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 270
    Illegal XbaI site found at 934
    Illegal PstI site found at 711
    Illegal PstI site found at 875
    Illegal PstI site found at 1670
    Illegal PstI site found at 2069
    Illegal PstI site found at 3165
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 702
    Illegal PstI site found at 711
    Illegal PstI site found at 875
    Illegal PstI site found at 1670
    Illegal PstI site found at 2069
    Illegal PstI site found at 3165
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 270
    Illegal XbaI site found at 934
    Illegal PstI site found at 711
    Illegal PstI site found at 875
    Illegal PstI site found at 1670
    Illegal PstI site found at 2069
    Illegal PstI site found at 3165
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 270
    Illegal XbaI site found at 934
    Illegal PstI site found at 711
    Illegal PstI site found at 875
    Illegal PstI site found at 1670
    Illegal PstI site found at 2069
    Illegal PstI site found at 3165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1195


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